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. 2010 Aug 3;285(41):31661–31671. doi: 10.1074/jbc.M110.145037

FIGURE 1.

FIGURE 1.

ROCK inhibitor Y-27632 alters the morphology of hematopoietic progenitors. HSPCs cultured on MSCs for 7 days in the absence (A, C, E, and G) or presence (B, D, F, and H) of Y-27632 were analyzed by scanning electron microscopy. A and B, untreated HSPCs (Control) can either be spherical (outlined arrowhead) or polarized (asterisk) with a uropod and/or lamellipodium (A), whereas Y-27632-treated HSPCs are mostly round (outlined arrowhead) or have one to three long and thin plasma membrane protrusions (filled arrowhead) (B). C–F, polarized HSPCs exhibit a uropod at the rear pole (C, outlined arrow), whereas most Y-27632-treated HSPCs do not (D). Y-27632-treated HSPCs have a narrower lamellipodium (D, filled arrow) in comparison to untreated cells (C and E, filled arrow). Large lamellipodia at the edge of thin protrusions are often observed in Y-27632-treated HSPCs (F, filled arrow). G and H, the surface of rounded HSPCs exhibits numerous microvillar-like structures (G) that are sensitive to the ROCK inhibitor (H).