FIGURE 3.

ROCK inhibitor Y-27632 alters the migration of hematopoietic progenitors. A, Transwell assay of untreated (Control) or Y-27632-treated HSPCs in the absence or presence of SDF-1α. The number of hematopoietic cells that migrated from the upper to the lower chamber after 1-h incubation is plotted as a percentage of total cells loaded (12.5 × 10⁴). Statistical analysis: t test. B, time-lapse video analysis of the migration of HSPCs. The arrow indicates the migrating front, whereas the outlined and filled white arrowheads point to the rear pole of HSPC present in the control or Y-27632-treated sample, respectively; the black arrowhead shows a third plasma membrane protrusion growing out from an Y-27632-treated HSPC. The elapsed time is shown in the upper left corner of each frame. Diagrams in the right panel depict the movement of 10 individual HSPCs for a period of 90 min. Scale bars, 15 μm.