TABLE 2.
Semi-quantitative assessment of ligand specificity of CBM60s determined by affinity gel electrophoresis of CBM60
Semi-quantitative binding is indicated as tight (+++), moderate (++), weak (+), and no significant interaction (−) depending on how far the proteins migrated on the gel. Polysaccharides that were not assessed are indicated by “ND.”
| Polysaccharide | Binding |
|
|---|---|---|
| CjCBM60A | vCBM60 | |
| Oat-spelt xylan | +++ | ND |
| Birchwood xylan | +++ | ND |
| Rye arabinoxylan | +++ | ND |
| Wheat arabinoxylan | +++ | + |
| Methylglucuronoxylan | +++ | ND |
| Carob galactomannan | − | − |
| Barley β-glucan | +++ | ++ |
| Lichenan | +++ | ND |
| Hydroxyethylcellulose | + | ND |
| Xyloglucan | +++ | ND |
| Laminarin | − | ND |
| Lupin galactan | +++ | ++ |
| Potato galactan | +++ | ++ |
| Pectic lupin galactan | +++ | ++ |
| Pectic potato galactan | +++ | ++ |
| Sugarbeet arabinan | − | ND |
| Debranched α1,5-arabinan | − | ND |
| Potato rhamnogalacturonan I | − | ND |