FIGURE 3.

Inhibition of Ras-dependent cell proliferation and survival by GATA-1. A, Ba/F3/N-RasE12/G1ERT cells were seeded at a density of 100/μl and cultured in RPMI supplemented with 1% FBS without IL-3 in the presence or absence of 1 μm 4-HT. Total numbers of viable cells were counted by trypan blue dye exclusion method on the indicated days. The results are shown as means ± S.D. of triplicate cultures. B, after 48 h of culture, DNA contents of 4-HT-treated or untreated cells were examined by propidium iodide staining. The proportions of cells in S-G2/M phase and subdiploid fraction are shown, respectively.