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p21CIP1/WAF1 but not p53 or p16INK4a/p19ARF mediates oncogenic Ras-induced suppression of erythropoiesis. A–C, LSK cells were isolated from BM of the indicated mice. After retrovirus infection, GFP+ cells were sorted and cocultured with MS-5 in the presence of rmSCF and rhEPO. The expression of CD45 and TER-119 was analyzed after 5 days. Bar graphs represent the relative TER-119+ cell numbers normalized to mock-transduced WT cells (dashed lines). n.s., not significant.
