TABLE 2.

Kinetic parameters of WT SET7/9 and the Y305F and Y245A mutants

Enzyme	TAF10 peptide substrate	Kma	kcata	kcat/Kma
		μm	min−1	μm−1min−1 × 103
WT	K189	160 ± 17	17 ± 0.62	110 ± 17
Y305F	K189	88 ± 5.0	17 ± 0.30	190 ± 11
Y305Fb	K189me1			11 ± 0.50
Y245A	K189	200 ± 35	0.53 ± 0.04	2.6 ± 0.47
Y245A	K189me1	210 ± 23	5.9 ± 0.23	28 ± 3.3
Y245A	K189me2	400 ± 29	6.5 ± 0.16	15 ± 1.2

^a Curve fitting errors were calculated from the hyperbolic fits for the Michaelis-Menten equation.

^b K_m and k_cat were not determined because the K_m value was beyond the measurable range; therefore, the k_cat/K_m value is reported.