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Product analysis of WT SET7/9 and the Y305F and Y245A mutants
| Enzyme | Quantity of enzyme | Measured product | Amount of product formeda |
|---|---|---|---|
| nmol | nmol | ||
| WT | 0.003 | Kme1 | 0.65 ± 0.07 |
| Y305F | 0.006 | Kme1 | 1.5 ± 0.49 |
| Kme2 | 0.033 ± 0.009 | ||
| Y245A | 0.100 | Kme1 | 0.80 ± 0.22 |
| Kme2 | 0.39 ± 0.021 | ||
| Kme3 | 0.076 ± 0.019 |
a Standard deviation was calculated from triplicate measurements.
