Cell and tissue distribution of Tmem123 mRNA. A, comparative analysis of Tmem123 mRNA expression levels between fresh DCs (LCs, SpDCs, and BMDCs), mature DCs, and two other cell lines (B16-F0 and WEHI164 cells). Fresh DCs (fLC, fSpDC, and fBMDC) were cultured for 24 or 48 h with or without GM-CSF (10 ng/ml) to induce maturation. B, Tmem123 expression in various tissues. C, Tmem123 mRNA expression in the CHS-elicited ear skin and control ear skin. The result from a representative experiment is shown. A, n = 2; B, n = 3; C, n = 3.