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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Nat Struct Mol Biol. 2010 Oct 3;17(10):1218–1225. doi: 10.1038/nsmb.1897

Figure 3.

Figure 3

DNA damage accumulation and DDR activation upon cellular aging. Expression of PCNA, Rpa32, Rad51, ATR, Rad17, Rad17-pS645, γ-ATM pS1981, Chk2-pT68, NBS1, NBS1S343ph, 53BP1, p53, p53S15ph, p21 and Cyclins A/B1/D1 in whole cell extracts of PD30 and PD75 HDFs. 75,000 cell equivalents were loaded per lane and equal loading was confirmed by ponceau staining prior to probing with primary antibodies. Hours after G1/S release are indicated and corresponding cell cycle phases have been determined by FACS analysis and indicated. γ-tubulin serves as loading control. Quantification of expression can be found in Fig. S5.