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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Nat Struct Mol Biol. 2010 Oct 3;17(10):1218–1225. doi: 10.1038/nsmb.1897

Figure 4.

Figure 4

Telomerase expression resets late passage cells to early passage cells. (a) Expression of H3, H4, SLBP, Asf1, H3K79mes and H4K20mes in synchronized cells from a young (left panel) and old individual (right panel). 75,000 cell equivalents were loaded per lane and equal loading was confirmed by ponceau staining prior to probing with primary antibodies. Hours after G1/S release are indicated and corresponding cell cycle phases have been determined by FACS analysis and indicated. γ-tubulin serves as loading control. (b) SILAC labeling of F9 and F92 HDFs and mass spectroscopy of histone proteins. Shown are percentages of newly synthesized histones (yellow) and histones from previous cell cycle (black). (c) Expression of 53BP1, Rpa32, SLBP, Asf1a/b, H3, H3K79me2, H4, H4K5ac and H4K20me2 in whole cell extracts of PD85 HDFs expressing catalytically active hTERT. 75,000 cell equivalents were loaded per lane and equal loading was confirmed by ponceau staining prior to probing with primary antibodies. Hours after G1/S release are indicated and corresponding cell cycle phases have been determined by FACS analysis and indicated. γ-tubulin serves as loading control.