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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Adv Genet. 2010;70:277–308. doi: 10.1016/B978-0-12-380866-0.60010-1

Table 1.

Some characteristics of genome-wide DNA methylation detection techniques

Techniquea Sensitivity nature of mCpGs detected Reference
RLGS 2–5 μg in Not1 sites Costello et al., 2000
MS-RDA 10 μg in restriction sites (e.g., HpaII) Ushijima et al., 1997
DMH 2 μg in restriction sites (e.g. HpaII, SmaI) Huang et al., 1999
MCA 5 μg SmaI restriction sites Estecio et al., 2007
McrBC 10 μg two CpGs separated by 55 bp to 3 kb Nouzova et al., 2004
MeDIP 2–4 μg all, CpG density-dependent Weber et al., 2005
MIRA 0.1–0.2 μg all, CpG-density-dependent Rauch et al., 2006
BS 5 μg all Lister et al., 2009
a

The techniques described are: RLGS, restriction landmark genomic scanning MS-RDA, methylation-sensitive representational difference analysis DMH, differential methylation hybridization McrBC, McrBC nuclease cleavage of methylated DNA MeDIP, Methylated DNA immunoprecipitation MIRA, Methylated-CpG island recovery assay BS, bisulfite sequencing