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. Author manuscript; available in PMC: 2011 Nov 15.
Published in final edited form as: Anal Biochem. 2010 Jul 27;406(2):214–221. doi: 10.1016/j.ab.2010.07.021

Figure 4. Relative expression of the target gene Tgfb1 is significantly influenced by reference gene stability following vocal fold mucosal injury.

Figure 4

Tgfb1 expression was normalized against reference genes Gapdh, Sdha, Sptbn1 and the geometric mean of Sdha and Sptbn1. Gene expression levels were calculated using the 2-ΔΔCT method and are presented as mean fold change ± standard error. n = 5 animals per time point. *, p < .01 versus both the non-injury control condition and Gapdh normalized condition at the same time point; d, day post-injury.