Skip to main content
. 2010 Oct 7;6(10):e1001149. doi: 10.1371/journal.pgen.1001149

Figure 5. In vivo effect of IntS overexpression on the excision of KplE1 prophage DNA.

Figure 5

A. Excision tests were performed in strain LCB6005 (tfaS::Km). pJFi and pBADintS encode wild-type TorI and IntS proteins, respectively. All in vivo excision experiments (see Materials and Methods) were performed in the presence of 1 mM IPTG for torI gene expression, and different amounts of arabinose were used for intS gene expression: 0% (lane 1 and 3), 0.00064% (lane 4), 0.0032% (lane 5), 0.016% (lane 6), 0.08% (lane 7) and 0.4% (lane 2 and 8). Excision efficiency is expressed as the ratio of ampicillin-resistant/kanamycin-resistant colonies (ApR/KmR). B. IntS relative amount (lanes 3–8 in reference to A.) in crude extracts was analyzed after separation on a 12% SDS-PAGE with Western blot using polyclonal IntS antiserum. The IntS position is indicated with an arrow.