Figure 5. Effect of cSPLUNC1 knock down on the development of OM induced by NTHI.

Chinchillas (3 per cohort) were administered either a control or cSPLUNC1 specific siRNA and challenged one day later with NTHI. The load of NTHI in middle ear effusions was subsequently determined two and four days after bacterial challenge. Knock down in cSPLUNC1 did not significantly impact survival of NTHI in the chinchilla middle ear under the conditions tested wherein a rigorous challenge dose was used.