Table 2. qPCR validation of 11 genes that were differentially regulated in the microarray analysis.

		RNA samples submitted to microarray and qPCR analysis							RNA samples submitted only to qPCR analysis
		ΔCta							ΔCta
Gene	Accession	Endometrium	Blood	Ste	ΔΔCtb	Foldchange	P	MCc	Endometrium	Blood	Ste	ΔΔCtb	Foldchange	P	MCc
PAG10	NM_176621	8.2	18.7	0.1	−10.4	1389	0.0003	Confirmed	7.5	19.1	1.2	−11.5	2964.0	0.002	Confirmed
GZMA	NM_001001142	6.8	16.5	0.9	−9.8	867	0.0112	Confirmed	7.7	16.1	1.5	−8.3	322.5	0.016	Confirmed
HMOX1	NM_001014912	12.4	16.8	0.1	−4.4	22	0.0002	Confirmed	11.9	16.0	1.3	−4.1	17.5	0.000	Confirmed
MRC1	XM_001252128	15.7	18.4	0.5	−2.7	6.7	0.0009	Confirmed	15.7	18.6	0.6	−3.0	7.8	0.065	Same direction
TSGA14	XM_583431	13.0	14.5	0.1	−1.5	2.9	0.0064	Confirmed	13.1	14.2	1.1	−1.1	2.1	0.523	Confirmed
CD16a	NM_001077402	10.1	11.0	0.2	−0.9	1.9	0.0115	Confirmed	11.4	11.1	1.4	0.3	1.2	0.889	Opposite direction
CD163	XM_613380	12.4	13.1	0.2	−0.7	1.6	0.0115	Confirmed	12.1	12.0	1.4	0.2	1.1	0.938	Opposite direction
PTX3	XM_614752	16.4	16.9	0.2	−0.5	1.4	0.0156	Opposite direction	17.4	16.4	0.6	1.0	2.0	0.351	Same direction
MCP1	NM_174006	15.2	15.3	0.1	0.0	1.0	0.0002	Opposite direction	15.4	14.5	2.3	0.8	1.8	0.814	Same direction
RNASE6	NM_174594	14.5	16.1	0.5	−1.6	3.0	0.1567	Same direction	11.1	14.2	1.0	−3.2	9.0	0.079	Same direction
SLCO2B1	NM_174843	15.8	18.7	0.1	−2.8	7.2	0.0887	Same direction	14.1	19.1	3.4	−5.0	31.3	0.365	Same direction

^aΔCt was calculated by the subtraction of the Ct value for the target gene from the Ct value for the housekeeping gene.

^bΔΔCt was calculated by the subtraction of the ΔCt value for the endometrium from the ΔCt value for the blood.

^cMC  =  Microarray confirmation.