Figure 5. AT3-UIM12 Binds with Ub Through Hydrophobic Interactions.

A, Mapping of the significantly perturbed residues by AT3-UIM12 interaction on Ub surface. The residues with chemical shift changes larger than the average have been labeled in pink and those larger than the average plus SD in red (ref. Fig. 3C). The Ub structure is referenced from the crystal structure (PDB code: 1UBQ). B, Intermolecular NOEs observed in the AT3-UIM12-Ub complex. A 1∶2 molar ratio of labeled AT3-UIM12 to unlabeled Ub were mixed for 3D ¹³C-filtered/¹⁵N-edited NOESY spectrum. The figure shows the NOE peaks projected onto the 2D plane. C, Mapping of the residues that have the intermolecular NOEs with Ub on the ribbon diagram of AT3-UIM12. D, Mapping of the residues that have the intermolecular NOEs with Ub on the surface diagram of AT3-UIM12, showing two patches for binding with two Ub molecules. The ribbon and surface representations were generated by MOLMOL.