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. 2010 Oct 7;5(10):e13202. doi: 10.1371/journal.pone.0013202

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Song et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A, Pull-down of K48- and K63-linked diUb by GST-fused UIM1, UIM2 or UIM12. The pull-down samples were detected by Western blotting with an anti-Ub antibody. B, SDS-PAGE of the GST-fused proteins (AT3-UIM1, AT3-UIM2 and AT3-UIM12) detected by Coomassie staining.