Skip to main content
. 2010 Oct 7;5(10):e13202. doi: 10.1371/journal.pone.0013202

Figure 8. Schematic Representation of the Tandem UIM Motifs with Various Forms of Ub Chains.

Figure 8

A, Non-cooperative binding mode. A representative for the mode is the tandem UIM motifs in Vps27p [16], which bind with Ub independently and non-cooperatively. B, Avid binding mode. The tandem UIM motifs in RAP80 bind with K63-linked diUb by forming a long α-helical structure [19]. The predicted linker becomes rather rigid and may be preferable to the configuration of K63-linked diUb. C, Cooperative binding mode. A short linker region (such as in AT3-UIM12) tends to be involved in the cooperative binding with Ub chains or ubiquitinated substrates. This cooperation is also likely to be occurred in poly- or multiUb. When the tandem UIM motifs bind with any form of Ub, a conformational arrangement and dynamics change occur in the linker region that may favor Ub binding. The dashed line indicates that either mono- or diUb is involved in the binding mode, and Ubi denotes different forms of Ub, standing for mono-, di-, poly- or multiUb.