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. 2010 Oct 7;6(10):e1001131. doi: 10.1371/journal.ppat.1001131

Figure 5. Slow inactivation of fusion machinery of DEN virions acidified in the absence of AL-containing membranes.

Figure 5

A. DiD-labeled DEN virions were pre-incubated at pH 5.5 for 15 or 30 min in the absence of liposomes or in the presence of PC liposomes (+PC LP); or at pH 4.5 for 30 min in the absence of liposomes. After low pH pre-treatment, virions without re-neutralization were mixed with LEM liposomes at pH 5.5. The initial rates of lipid mixing (black bars) and the extents of lipid mixing at t = 10 min (gray bars) were normalized to those between DEN and LEM liposomes observed without low pH-preincubation (Control). B. MA104 cells with pre-bound DiD-labeled DEN were pre-treated or not pre-treated at 37°C with pH 6.0 or 5.5 medium (15 min, 37°C). Low pH application was followed by a 40-min incubation in the complete medium at 37°C. Viral fusion events along the endocytic pathway were detected as an increase in the mean total fluorescence per imaging field. Control – no low pH pre-treatment, taken as 100%. A, B. The data are presented as mean +/- s.d. (n = 3).