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. 2010 Oct 7;6(10):e1001132. doi: 10.1371/journal.ppat.1001132

Figure 5. Phenotypic analysis of parasites expressing FH2 domains.

Figure 5

(A) Plaque assays on transgenic parasites upon stabilization of FH2 after 6 days. The scale bar represents 1 mm. (B) The area of 30 plaques formed by the individual strains ± Shld-1 were measured by using the ImageJ program. Values are means ± SD. Statistical significance was evaluated using the unpaired t test. ***P<0.0001. (C) Egress assay on transgenic parasites upon stabilization of FH2 for 63 hrs. Egress was triggered by 3 µM of A23187. The extent of vacuole lysis and parasite spreading were scored by visual examination on IFA. Values are means ± SD for three independent experiments. ***P<0.0001. (D) Invasion assay on transgenic parasites upon stabilization of FH2 for 63 hours. Parasites were allowed to invade new HFF cells for 24 hours. Values are means ± SD for three independent experiments. ***P = 0.0007, ***P<0.0001, ***P<0.0001 respectively. (E) Parasites were treated as in (C) and monitored for trail deposition in gliding assay using SAG1 antibodies. No difference in trail deposition was seen for RHΔhxgprt strain upon Shld-1 treatment. Values (n = 600 parasites) are means ± SD for three independent experiments. **P = 0.0046, ***P = 0.0008, ***P = 0.0002 respectively. (F) Phenotypic analysis of circular gliding movement in live gliding parasites; error bars represent standard deviation. Values (n = 30 parasites) are means ± SD for three independent experiments. ***P = 0.0007, ***P = 0.0007, ***P<0.0001, ***P<0.0001 respectively. (G) Phenotypic analysis of helical gliding movement in live gliding parasites; error bars represent standard deviation. All data of this figure represent mean values of three experiments. Values (n = 30 parasites) are means ± SD for three independent experiments. ***P<0.0001, ***P<0.0001, **P = 0.0025, **P = 0.0025 respectively.