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. 2010 Oct 7;6(10):e1001136. doi: 10.1371/journal.ppat.1001136

Figure 4. Influenza viruses differentially activate LTGF-β.

Figure 4

rLTGF-β (10 ng/ml) was incubated alone (white bar) or with different influenza virus subtypes (90,000 RFU) for 1 h at 37°C and TGF-β activity measured by PAI/L assay (A) or ELISA (B). (C) rLTGF-β (0.4 µg) was incubated with PBS, bNA, N4 Virus (2 µg), or HK/483 virus (HK/483, 2 µg) in the presence or absence of 1 µM oseltamivir carboxylate (NAi) for 1 h at 37°C. Proteins were separated by SDS-PAGE under reducing conditions, transferred to nitrocellulose, and probed with anti-LAP antibody by Western blot analysis. Error bars represent standard error of the mean.