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. Author manuscript; available in PMC: 2010 Oct 7.
Published in final edited form as: Stem Cells. 2007 Jul 5;25(10):2551–2558. doi: 10.1634/stemcells.2006-0745

Figure 2.

Figure 2

Generation of rFasL-expressing mouse ES cell clones. CE1 or D3 ES cells were transfected with the control plasmids CE3 [14] and Xgfp or the rFasL-expressing plasmids EF and XF, respectively. (A): Multiple ES cell clones were analyzed by reverse transcription-polymerase chain reaction for endogenous mFasL (242 base pairs) and the recombinant rFasL (212 base pairs) expression. From these ES cell lines, clone ES.XF1 had the highest rFasL transcription. (B): Functional FasL expression was analyzed in Jurkat cell assays. In at least three independent experiments, there was no significant effect of any of the rFasL-transfected ES cell lines on the induction of Jurkat cell apoptosis. (C): Western blot experiments using the rabbit anti-mouse FasL antibody N-20 demonstrated multiple bands including a band at 32 kDa, which was also present in rat thymus lysates. Abbreviations: EF, pCE-rFasL-IRES-EGFP; ES, embryonic stem; mFasL, mouse Fas ligand; rFasL, rat Fas ligand; XF, pCX-rFasL-IRES-EGFP.