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Migrating primordial germ cells (PGCs) were identified by vasa expression using whole mount in situ hybridization of wild-type and dnd-MO injected (2,000–4,000 pg/embryo) embryos at the embryonic shield to larval stages. (A–E and F–J) PGCs of wild-type and dnd morphants, respectively. (A and F) Embryonic shield stage. (B and G) Two- to four-somite stage. (C and H) Twenty-somite stage. (D and I) Thirty-somite stage. (E and J) Larvae at 5 d postfertilization. (Scale bars, 500 μm.)
