Fig. 2.

Delivery of icv leptin is restricted to the brain in STZ-induced insulin-deficient mice. Phosphorylated STAT3 (P-STAT3), STAT3, and β-actin (used as a loading control) protein levels (A) and Pomc, Npy, and Agrp mRNA contents (B) were assessed in the hypothalamus of T1D-icv-PBS and T1D-icv-Leptin-50 mice shown in Fig.1A. (C) Plasma leptin levels were measured in mice shown in Fig. 1A. (D) P-STAT3, STAT3, and β-actin (used as a loading control) protein levels were assessed in the liver of mice shown in Fig 1A. Error bars represent SEM. Statistical analyses were done using a two-tailed unpaired Student's t test or one-way ANOVA (Tukey's posttest) when two or three groups were compared, respectively (n = 5–7 in each group). *P < 0.05; **P < 0.01; ***P < 0.001 vs. T1D-icv-PBS mice. NS, not statistically different.