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SUMO substrates are enriched in hpSAS. (A) The SAS consists of a hydrophobic residue (Ψ), the lysine residue to which SUMO is attached (K), any amino acid (x), and an acidic amino acid (D or E). An hpSAS (≥91%) is shown (I/V/LKx D/E). (B) Proteins identified by yeast two-hybrid screening using the SCE as bait are enriched in hpSAS. Whereas 40% of the Arabidopsis proteome contains hpSAS, 70% of SCE interactors contain hpSAS.
