Fig. 5.

Effects of oryzalin on bidirectional movement of CESA complexes in the T165E line. Time-lapse confocal images of YFP::CESA6 in hypocotyl cells of 2-d-old etiolated plants grown at 30 °C were used to measure CESA particle velocity. u and d represent two opposite directions of particle movement relative to the major axis (Fig. S4 A and B). (A–C) Control treatment with 0.02% methanol or DMSO at 30 °C for 2 to 3 h. (D–F) Treatment with 20 μM oryzalin at 30 °C for 2 to 3 h. (A and D) Representative kymograph displays effects of oryzalin treatment on bidirectional particle translocation. (B and E) Average time projections of 61 frames representing 5 min. (C and F) Histograms of particle velocities calculated from kymograph analysis of 100 to 200 particles from three to six cells of a single seedling. (Scale bars: 10 μm in B and E.)