Fig 1.

Effects of pharmacological inhibitors on DENV replication. DENV replicon RNAs were introduced into Huh-7.5 cells. At 24 h postelectroporation, the cells were treated with the indicated concentrations of Cerulenin (A) and C75 (B), maintained for 24 h, and assayed for luciferase activity. RLU, relative luciferase units. Huh-7.5 cells were DENV-infected [multiplicity of infection (MOI) = 1] for 4 h and then treated with the indicated C75 concentrations. Twenty-four hours postinfection, viral RNA levels (C) or released virus (D) was quantified along with cellular ATP levels. The dotted line indicates the limit of detection. Baby hamster kidney cells were infected with YFV 17D (E; MOI = 1), or Vero cells were infected with WNV NY99 (F; MOI = 1) and treated as above. Data represent the average ± SEM.