Table 1.

Native porcine cardiac myosin and human WT-RLC exchanged and FHC-RLC mutant exchanged myosins have similar unloaded kinetics and mechanics

	Native	WT	N47K	R58Q
Unloaded velocity (μm/s)	0.80 ± 0.06	0.79 ± 0.03	0.78 ± 0.04	0.80 ± 0.05
Vmax for regulated thin filaments (μm/s)	1.14 ± 0.04	1.08 ± 0.05	1.06 ± 0.05	1.06 ± 0.05
pCa50 for regulated thin filaments	6.33 ± 0.04	6.19 ± 0.05	6.26 ± 0.04	6.21 ± 0.05

There are no significant differences between mutant and WT exchanged myosins.