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. 2010 Sep 16;107(40):17421–17426. doi: 10.1073/pnas.1008491107

Fig. 2.

Fig. 2.

The CRN protein family forms a class of modular proteins that are translocated inside host cells. (A) Schematic representation of CRN2, CRN8, and CRN16 N-terminal domains identified in P. infestans and used for translocation assays presented. (B) Virulence assessment of transgenic P. capsici strains on wild-type and transgenic R3a N. benthamiana leaves. Infection rates of transgenic strains carrying empty vector (pTOR), CRN2:AVR3a, CRN16:AVR3a, and their mutated counterparts (CRN2mut:AVR3a, CRN16mut:AVR3a) were determined on wild-type and R3a leaves 4 dpi. Values represent the mean percentage of successful infections over three independent experiments. (C) Representative pictures showing wild-type and transgenic R3a leaves inoculated with strains carrying (Left to Right) the empty vector pTOR, CRN2:AVR3a, and CRN16:AVR3a as well as their mutated counterparts CRN2mut:AVR3a and CRN16mut:AVR3a. Pictures were taken 4 dpi. Crn-Avr3a expression was confirmed by RT-PCR analyses (Fig. S2). Lesions are marked by circles.