Fig. 5.
Fibronectin contributes to plakoglobin-dependent inhibition of cell motility. (A) Representative tracks (n=10) showing the decrease in motility of PG−/− cells upon introduction of PG by adenovirus as compared to GFP-only control. (B) Western blot analysis of FN expression in the whole cell lysates of PG+/−, control (GFP) and PG-transduced PG−/− cells. (C) Immunofluorescence staining showing restoration of FN expression and deposition in PG−/− cells transduced with adenovirus expressing PG (green) compared with GFP-expressing control cells. Scale bar: 20 μm. (D) Representative tracks (n=10) showing the motile behavior of PG−/− keratinocytes plated onto plastic- (no predeposited matrix), fibronectin (FN)- and laminin-332 (LN-332)-coated dishes.