Fig. 6.

Plakoglobin increases fibronectin RNA levels through increasing its stability. (A) qRT-PCR analysis of the FN RNA, which is significantly decreased in PG^−/− cells as compared with controls (experiment done in triplicate with two sets of primers and normalized to Gapdh; error bars represent ± s.d.). (B) Luciferase activity analysis shows an increase in FN promoter activity in PG^−/− cells, suggesting that PG does not increase FN levels through promoter activation (Firefly luciferase activity normalized to Renilla activity used as a transfection efficiency control; experiment done in quadruplicate, error bars represent ± s.d.). (C) FN RNA stability analysis in PG^+/− and PG^−/− cells treated with actinomycin D (2 μg/ml) for the specified times. Samples from treated cells were normalized to their matching PG^+/− and PG^−/− controls (time 0), both of which were given a relative value of 1 (experiment done in triplicate with two different sets of primers; error bars represent ± s.d.).