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. 2010 Sep 28;123(20):3587–3595. doi: 10.1242/jcs.073817

Fig. 5.

Fig. 5.

CMF70 is required for normal flagellar motility in T. brucei. (A) qRT-PCR analysis shows a dramatic decrease in cmf70 mRNA in CMF70-knockdown cells (+tet) compared with control cells (−tet). (B) Growth curves of uninduced (−Tet) and induced (+Tet) CMF70-knockdown cells show slowed growth within 24 hours of RNAi induction. Each curve represents an average of two independent cell counts and the error bars indicate s.d. (C) Sedimentation assays (Bastin et al., 1999; Ralston et al., 2006) on uninduced (−Tet) versus induced (+Tet) CMF70 RNAi cells show that CMF70-knockdown cells sediment, indicative of a motility defect. The curves represent an average of two independent measurements and the error bars indicate s.d. (D) Motility traces of uninduced (−tet) and induced (+tet) CMF70 RNAi cells. Lines trace the movement of individual cells over a 35 second time interval. Numbers in each panel represent individual cells. Scale bars: 50 μm.