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. 2010 Sep 28;123(20):3587–3595. doi: 10.1242/jcs.073817

Fig. 6.

Fig. 6.

Loss of CMF70 suppresses flagellar paralysis of central pair knockdowns. (A) qRT-PCR analysis of pf16 and cmf70 transcripts in PF16 single-knockdown or PF16–CMF70 double-knockdown cells. Expression of RNAi-targeted transcripts in tetracycline-induced (+ Tet) cultures is shown relative to uninduced controls (−Tet). Averages of two independent experiments are shown with error bars representing s.d. (B) Analysis of flagellar beating in PF16 single-knockdown (n=55), PF16–CMF70 double-knockdown (n=50) and control cells (n=57). Beats at the tip of the flagellum were quantified over a 10 second interval, as described (Ralston et al., 2006).