Table 2.
Hoechst staining and flow cytometry showing apoptosis of hepatocellular carcinoma cell lines and normal liver cell line (mean ± SD)
| Cell line |
Apoptotic cells (%) |
F-value | P-value | |||
| Control | Ad.IL-24 | SG600-EGFP | SG600-IL24 | |||
| Hoechst staining | ||||||
| HepG2 | 2.5 ± 0.1 | 4.0 ± 0.3 | 5.5 ± 0.3 | 42.0 ± 4.5 | 156.6 | 0.000007 |
| Hep3B | 3.0 ± 0.2 | 3.5 ± 0.3 | 4.5 ± 0.2 | 56.0 ± 3.8 | 202.4 | 0.000003 |
| MHCC97L | 1.6 ± 0.2 | 3.0 ± 0.4 | 2.6 ± 0.3 | 40.5 ± 1.9 | 143.2 | 0.000010 |
| L02 | 1.0 ± 0.1 | 1.2 ± 0.2 | 1.4 ± 0.1 | 2.0 ± 0.1 | 1.78 | 0.360 |
| Flow cytometry | ||||||
| HepG2 | 2.0 ± 0.1 | 4.2 ± 0.4 | 10.0 ± 2.0 | 56.5 ± 4.0 | 203.4 | 0.000003 |
| Hep3B | 2.5 ± 0.2 | 5.0 ± 0.2 | 13.5 ± 1.5 | 78.3 ± 3.5 | 313.2 | 0.000001 |
| MHCC97L | 1.4 ± 0.2 | 3.2 ± 0.4 | 10.4 ± 1.0 | 43.3 ± 2.5 | 160.6 | 0.000006 |
| L02 | 1.0 ± 0.1 | 1.2 ± 0.1 | 1.4 ± 0.1 | 1.7 ± 0.1 | 1.62 | 0.280 |