. 2010 Oct 7;16(37):4685–4690. doi: 10.3748/wjg.v16.i37.4685

Table 1.

Conditions and products of polymerase chain reaction followed by restriction fragment length polymorphism

Gene	Primers	Tm (°C)	RE	bp	Alleles
ApoB	Forward: GGAGACTATTCAGAAGCTAA	55	XbaI	710	X-: 710 bp
ApoB	Reverse: GAAGAGCCTGAAGACTGACT	55	XbaI	710	X+: 433 and 277 bp
ApoE	Forward: ACAGAATTCGCCCCGGCCTGGTACAC	60	HhaI	252	E2: 91 and 84 bp
	Reverse: TAAGCTTGGCACGGCTGTCCAAGGA				E3: 91, 48 and 36 pb
					E4: 72, 48 and 36 bp
CYP7A1	Forward: CAGAGCATGGACAGGGAGCAG	55	BsaI	948	A: 581, 367 bp
CYP7A1	Reverse: GCAACTCCTCATGGCTGAGGTT	55	BsaI	948	C: 542, 367 and 39 bp

Forward and reverse primers in 5’ to 3’ directions. Tm: Annealing temperature; RE: Restriction enzyme.