Figure 4.

Kinetics of colocalization of chitosan–plasmid DNA polyplexes and lysosomes following transfection. Colocalization coefficients between labeled polyplexes and fluorescent dextran-labeled lysosomes were calculated from confocal live-cell images of multiple cells taken at specific time points following transfection using the LSM 510 colocalization analysis module. The analysis reveals an initial accumulation of the polyplex in the lysosomes followed by a time-dependent lysosomal escape from 12 to 48 hours that was similar for all chitosans. Results shown are the average of three (N = 3) independent experiments ± SD where each experiment quantified a minimum of 15 cells with three optical sections each.