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. 2010 Jun;89(6):597–602. doi: 10.1177/0022034510363363

Figure 2.

Figure 2.

Luciferase assays performed on MD10-A2 cells transiently co-transfected with NMA/BAMBI-V5-His expression construct and the DSPP-Luc reporter construct. (A) Luciferase assay demonstrating the effects of TGFβ on DSPP expression in MD10-A2 cells. TGFβ significantly reduced expression of DSPP, as indicated by the asterisk (*) (n = 6; p = 0.05; ± SEM). (B) Luciferase assay demonstrating the effects of Nma/BAMBI on DSPP expression in MD10-A2 cells. Nma/BAMBI significantly reduced expression of DSPP, as indicated by the asterisk (*) (n = 6; p = 0.05; ± SEM). (C) Luciferase assay demonstrating that overexpression of Nma/BAMBI had a negative effect on DSPP promoter activity compared with the reverse-oriented control construct Nma R. While double transfections appeared to decrease the intensity of the reporter construct, expression of Nma/BAMBI-V5-His (Nma F) significantly down-regulated DSPP promoter activity compared with Nma R Control Vector (n = 6; p = 0.05; ± SEM). (D) Luciferase assay demonstrating the effects of TGFβ on MD10-A2 cells transfected with Nma/BAMBI-V5-His expression construct. TGFβ lifted Nma/BAMBI’s negative effect on DSPP expression, but not at a statistically significant level (n = 6; p = 0.05; ± SEM).