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. 2010 Jun;89(6):597–602. doi: 10.1177/0022034510363363

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Figure 3. — Quantitative real-time PCR (QRT-PCR) and ALP staining of MD10-A2 cells stably transfected with NMA/BAMBI-V5-His expression construct in the forward orientation vs. non-transfected MD10-A2 cells cultured in control media. (A) The average fold change in expression of Nma/BAMBI, DMP1, and DSPP in MD-10A2 cells overexpressing Nma/BAMBI is shown. Nma/BAMBI was expressed four-fold compared with non-transfected control cells. Additionally, DMP1 expression was up-regulated four-fold, while DSPP expression was down-regulated 66%. (B) Control non-transfected MD10-A2 cells showing diffuse uniform ALP staining. (C) MD10-A2 cells overexpressing Nma/BAMBI show positive ALP staining (black arrow) associated with the formation of large multi-layered cell nodules.