kiss1r mRNA transcripts are present in several A. burtoni tissues and brain regions. Semiquantitative gel analysis of PCR products from cDNA reverse transcribed using RNA from whole brain (Br), olfactory bulbs (Ob), pituitary (Pi), retina (Re), saccule (Sa), gill (Gi), kidney (Kd), spleen (Sp), stomach (St), intestine (In), liver (Li), skeletal muscle (Mu), heart (He), testis (Te), ovary (Ov), telencephalon (TEL), hypothalamus and preoptic area (HYP), midbrain (MB), cerebellum (CE), hindbrain (HB), and spinal cord (SC). Negative control (Neg) column is the PCR without template tissue cDNA. Upper rows are PCR products obtained with A. burtoni kiss1r-specific primers, and lower rows are products from actin-specific primers from the same tissue samples.