Table 4.

Projection-Reconstruction Experiments

Measurement	Sample	Experiment(s)†	Reconst. Alg.‡	Time (hrs.)	Ref
Backboneassignment	1mMubiquitin	(3,2)-D HNCO	LV	0.49	[53]
Backboneassignment	1mMubiquitin 1.7 mM HasA	(3,2)-D HNCA, HN(CO)CA,HNCO	LV	1.5 0.56	[141]
Backboneassignment	1mMubiquitin	(4,2)-D HNCOCA	LV	0.63	[136]
Backboneassignment	3mMnucleaseA inhibitor	(4,2)-D HNCOCA	LV, BP	0.58	[137]
Backboneassignment	2mMproteinGB1 domain	(5,2)D HACACONH	LV	1.45	[43]
Backboneassignment	3 mMnucleaseA inhibitor	(3,2)-D HNCA	LV, BP	1.0	[70]
Backboneassignment	0.94 mM HCA II 0.9 mMcalbindin D28K	(4,2)-D HNCACB, HN(CO)CACB, intra- HNCACB, HNCACO, HNCOCA, HNCOi-1CAi HACA(CO)NH, HACANH	LV, BP, HBLV	307.2	[72]
Backboneassignment	1.7 mM HasA	(3,2)-D HNCO	BP with CLEAN	0.83	[138]
Backboneassignment	1.7 mM HasA	(3,2)-D HNCO	LV, BP, Others	not given	[152]
Sidechainassignments	1mMproteinGB1 domain	(4,3)-D HC(CCO)NH and intra-HC(C)NH	LV	47.5	[142]
NOESY	0.9 mM HCA II	(4,2)-D methyl-amide NOESY detecting CH3 NH	FBP	88	[71]

^†(n,m)-D indicates that n dimensions of correlations are collected with m independent evolution times. Underlining indicates coevolved nuclei; underlined nuclei grouped in {curly braces} indicate independent sets of coevolved nuclei. (Parentheses) indicate nuclei through which magnetization is passed, without evolving chemical shifts. intra- indicates that only the intraresidue peaks are detected. The names for some experiments have been adjusted from the original publications to maintain consistency in nomenclature.

^‡LV = lower-value, BP = backprojection, HBLV = hybrid backprojection/lower-value, FBP = filtered backprojection. Ridge and Mandelshtam’s method [73] has not been demonstrated on experimental data. Yoon and coworkers reported several methods which we have classified as “iterative model-fitting” and which are discussed in that section.