Table 2.

Phenotypic differences between wild-type E. coli O157:H7 (43894) and the isogenic pO157-cured strain (43894-Cu)

Mode of Action	Chemical	43894	43894-Cu
Nitrogen source	L-Arginine, L-Phenylalanine, L-Tryptophan	−	+
	D-Lysine, D-Galactosamine	+	−
	L-Serine	++	−
Peptide Nitrogen source	Ala-His	++	−
	Gly-His, His-Asp, His-Leu, Ile-Phe, Ile-Trp, Ile-Tyr, Leu- Asp, Leu-Ile, Leu-Leu, Leu-Phe, His-Glu, His-His, Ile-Leu, Leu-His, Leu-Tyr, Met-Tyr, Phe-Met, Phe-Tyr, Trp-Val, Tyr- lle, Tyr-Val, Leu-Leu-Leu	−	+
	Leu-Val, Met-Ile, Met-Leu, Met-Val, Phe-Ile, Trp-Leu, Trp- Tyr, Tyr-Leu, Tyr-Tyr, Val-Ile, Val-Leu, Gly-Gly-Phe	−	++
	Leu-Trp, Trp-Lys, Trp-Phe, Trp-Trp	−	+++
	Lys-Leu, Lys-Ser, Lys-Thr	+	−
	Phe-Trp, Thr-Leu, Val-Asp	+	+++
Phosphorous source	Thiophosphate, Dithiophosphate	−	++
	Cysteamine-S-Phosphate	+	−
	Pyrophosphate	+	+++
Sulfur source	L-Cysteine	+	+++
pH, deaminase	pH 9.5 + L-Aspartic Acid	−	+
Chemicals	Cefazolin, Cefoxitin, Piperacillin, Alexidine, Diamide, Chloroxylenol, Josamycin, Disulphiram, Phenethicillin, Orphenadrine	−	+
	Amikacin, Bleomycin, 1-Chloro-2,4-Dinitrobenzene, Methyltrioctylammonium, Chloride, Harmane, Ceftriaxone	−	++
	Colistin, Tetracycline, Penimepicycline, 2-Nitroimidazole, Lithium Chloride, Blasticidin S, Tolylfluanid	+	−
	Streptomycin, Rifamycin SV	+	+++
	Chromium Chloride	++	−
	Chloramphenicol	+++	+

⁻bacteria grew the same or less than the negative control which was a well without substrate

⁺bacteria grew 1- to 2-fold more than the negative control

⁺⁺bacterial grew 2- to 3-fold more than the negative control

⁺⁺⁺bacteria grew >3-fold more than the negative control