Table 3.

Phenotypic differences between wild-type E. coli O157:H7 (Sakai) and the isogenic pO157-cured strain (Sakai-Cu)

Mode of Action	Chemical	Sakai	Sakai-Cu
Peptide Nitrogen source	Leu-Glu, Gly-Gly-D-Leu	+	−
	Met-Lys	−	++
Nutritional supplement	L-Cysteine	−	+
Phosphorous source	Pyrophosphate	+	+++
Chemicals	Lincomycin, Cloxacillin, Enoxacin, Ceftriaxone, Ofloxacin, Spiramycin, Cefoxitin, Chloramphenicol, ,7-Dichloro-8- hydroxyquinoline, 5-Chloro-7-Iodo-8- Hydroxyquinoline, Dichlofluanid, Cefamandole, Methyltrioctylammonium Chloride, Harmane, Chlorhexidine, Disulphiram, Proflavine, Dodine, Oxytetracycline, Tolylfluanid	−	+
	Amoxicillin, Colistin, Nafcillin, Dodecyltrimethyl Ammonium Bromide, Cefuroxime, 9-Aminoacridine, Chelerythrine, Cefmetazole, Cetoperazone, Thiamphenicol, Pipemidic Acid, Antimony (III) chloride, Josamycin	−	++
	Penicillin G	−	+++
	Vancomycin, Hygromycin B	+	−
	Cephalothin, Oxolinic acid, Moxalactam, Acriflavine, Sodium Orthovanadate, Cefamandole, Iodonitro Tetrazolium Violet, Orphenadrine	+	+++
	Nitrofurazone, Poly-L-lysine, Tinidazole, Ornidazole	++	−
	Apramycin	+++	−

⁻bacteria grew the same or less than the negative control which was a well without substrate

⁺bacteria grew 1- to 2-fold more than the negative control

⁺⁺bacterial grew 2- to 3-fold more than the negative control

⁺⁺⁺bacteria grew >3-fold more than the negative control