Table 4.

Metabolic capacities of wild-type E. coli O157:H7 strains

Mode of Action	Substrate(s)	43894	Sakai	WSU180
Carbon source	α-Keto-Butyric Acid, α-Hydroxy-Butyric Acid, L-Galactonic Acid-g-Lactone	+	+++	++
	Dihydroxy-Acetone	+	+++	+++
Nitrogen source	D-Lysine, D-Galactosamine, Thymidine	+	−	−
	L-Glutamic Acid	+	−	+
	Guanine	+	+	−
	L-Alanine	++	−	+
Phosphorus Source	Tripolyphosphate	+	+	−
	2-Aminoethyl Phosphonic Acid	++	+	−
Sulfur source	Lanthionine	+	−	++
Nutritional supplement	L-Isoleucine + L-Valine, L-Leucine, D;L-Diamino- Pimelic Acid, Nicotinic Acid, L-Glutamic Acid, Pyridoxamine	−	+	−
	Quinolinic Acid, Inosine + Thiamine, α-Keto-Butyric Acid, D;L-Carnitine, Choline	+	+	−
Dipeptide Nitrogen source	Leu-Val	−	−	+
	Tyr-His	−	+	−
	Trp-Lys, Trp-Phe, Trp-Trp	−	+	++
	Trp-Tyr	−	++	+
	Tyr-Tyr	−	++	++
	Ala-His, Ala-Lys, Arg-Asp, Arg-Leu, Arg-Ser, Arg- Val, His-Pro, Ile-Ala, Gly-Leu, Ile-Arg, Leu-Arg, Leu-Gly, Lys-Gly	+	−	−
	Ile-Gln, Gly-Arg, Ala-Tyr, Leu-Ala, Lys-Ser, Ala-lle	+	−	+
	Ile-Ser, Gly-Thr	+	+	−
	Ala-His, Pro-Lys, Val-Gln, Val-Ser	++	−	−
	His-Ala, Pro-Val	++	−	+

⁻bacteria grew the same or less than the negative control which was a well without substrate

⁺bacteria grew 1- to 2-fold more than the negative control

⁺⁺bacterial grew 2- to 3-fold more than the negative control

⁺⁺⁺bacteria grew >3-fold more than the negative control