Table 5.

Osmotic, pH, and chemical sensitivities of wild-type E. coli O157:H7 strains

Mode of Action	Substrate(s)	43894	Sakai	WSU180
Osmotic sensitivity	4% Sodium Lactate	+	−	−
pH, decarboxylase	pH 4.5 + L-Norvaline	+	−	−
	pH 4.5 + a- Amino-N-Butyric Acid	+	−	−
	pH 4.5 + 5-Hydroxy-L-Lysine	+	−	−
	pH 4.5 + Urea	+	−	−
	pH 4.5 + b-Hydroxy Glutamate	+	−	+
	pH 4.5 + D,L Diamino-Pimelic Acid	+	+	−
pH, deaminase	pH 9.5 + Agmatine	+	−	+
	pH 9.5 + L-Homoarginine	+	+	−
Chemical sensitivity	Cefoxitin, Disulphiram, Methyltrioctylammonium Chloride, Polymyxin B	−	−	+
	Ciprofloxacin	−	+	−
	Chloroxylenol, Orphenadrine, Oxolinic acid	−	+	+
	1-Chloro-2,4-Dinitrobenzene Piperacillin, Sodium Orthovanadate	−	+	++
	Furaltadone	−	++	−
	2- Phenylphenol	−	+++	−
	2,2`-Dipyridyl, 2-Nitroimidazole, 3, 4- Dimethoxybenzyl alcohol, 5,7-Dichloro-8- hydroxyquinoline, 5-Fluoro-5'-deoxyuridine, Captan, Capreomycin, Cefmetazole, Cupric chloride, D,L- Methionine, Hydroxamate, D,L-Serine, Hydroxamate, D-Cycloserine, Gallic Acid, Lithium Chloride, Neomycin, Phleomycin, Procaine, Sodium Nitrite, Tobramycin, Tolylfluanid, Umbelliferone	+	−	−
	2,4-Dintrophenol, Blasticidin S, Colistin, Proflavine, Thioridazine, Thiosalicylate, Trifluoperazine	+	−	+
	18-Crown-6-Ether, Cobalt chloride, L-Aspartic-b- Hydroxamate, Sanguinarine	+	+	−
	Tinidazole	+	++	−
	Amoxicillin, Chlorhexidine, Chromium Chloride, Guanidine hydrochloride, Hydroxylamine, Phenyl- Methyl-Sulfonyl-Fluoride (PMSF), Protamine Sulfate, Sisomicin	++	−	−
	Ethionamide	++	−	+
	Geneticin (G418)	++	−	+
	5-Azacytidine, Semicarbazide hydrochloride, Troleandomycin	++	+	−
	Aluminum Sulfate, Dihydrostreptomycin	+++	−	−
	Kanamycin	+++	+	−
	Zinc chloride	+++	+	+

⁻bacteria grew the same or less than the negative control which was a well without substrate

⁺bacteria grew 1- to 2-fold more than the negative control

⁺⁺bacterial grew 2- to 3-fold more than the negative control

⁺⁺⁺bacteria grew >3-fold more than the negative control