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. Author manuscript; available in PMC: 2011 Jan 29.
Published in final edited form as: Mol Cell. 2010 Jan 29;37(2):196–210. doi: 10.1016/j.molcel.2009.12.034

Figure 2. Dissociation of post-TCs by ABCE1.

Figure 2

(A) Left panel - scheme for purification of ABCE1 from RRL, right panel - purified ABCE1 resolved by SDS-PAGE. (B-F) Dissociation of pre-TCs, assembled on MVHL-STOP mRNA with [32P]60S subunits and (B-E) native or (F) transcript Leu-tRNALeu, after incubation with different combinations of eRFs, ABCE1, eIFs and unlabeled 60S subunits at indicated Mg2+ concentrations, assayed by SDG centrifugation. Upper fractions were omitted for clarity.