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. Author manuscript; available in PMC: 2011 Jan 29.
Published in final edited form as: Mol Cell. 2010 Jan 29;37(2):196–210. doi: 10.1016/j.molcel.2009.12.034

Figure 3. Dissociation of tRNA and mRNA from recycled 40S subunits.

Figure 3

(A-D) Ribosomal association of (A) [32P]tRNALeu and (B-D) [32P]MVHL-STOP mRNA after incubating pre-TCs with eRFs, ABCE1 and eIFs at 2.5 mM Mg2+, as indicated, assayed by SDG centrifugation. (E, F) Toe-print analysis of ribosomal complexes, obtained by incubating pre-TCs, assembled on MVHL-STOP mRNA using native Leu-tRNALeu, with combinations of eRFs, ABCE1 and eIFs at indicated Mg2+ concentrations. Lanes C, T, A, G depict cDNA sequences corresponding to MVHL-STOP mRNA. The positions of full-length cDNA and of toe-prints corresponding to ribosomal complexes are indicated. (G) Influence of ABCE1 on association of [32P]eRF1 with post-TCs at different Mg2+ concentrations, assayed by SDG centrifugation. Upper fractions were omitted for clarity from panels A, G.