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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Arch Biochem Biophys. 2010 May 9;499(1-2):62–68. doi: 10.1016/j.abb.2010.05.007

Table 2. Normal specific enzyme activities of non-targeted enzymes malic enzymes and ATP citrate lyase in cell lines with various knockdown SCOT activities and glucose-stimulated insulin release.

Enzyme activities are expressed as nmol product/min/mg whole-cell supernatant fraction protein and are the mean ±SE of (N) replicate measurements.

Cell line Malic Enzyme ATP Citrate Lyase
Activity % of Control Activity % of Control
832/13 77 ±10 (3) 100 129 ±8 (8) 100
CHS 79 ±11 (3) 103 137 ±12 (6) 106
SCOT 68 99 ±9 (3) 130 123 ±6 (4) 95
SCOT 713 73±7 (3) 95 134 ±13 (4) 104
SCOT 1676 72 ±10 (3) 93 104 ±11 (10) 81
SCOT 1184 59 ±7 (3) 77 106 ±3 (6) 82