Table 2. Normal specific enzyme activities of non-targeted enzymes malic enzymes and ATP citrate lyase in cell lines with various knockdown SCOT activities and glucose-stimulated insulin release.
Enzyme activities are expressed as nmol product/min/mg whole-cell supernatant fraction protein and are the mean ±SE of (N) replicate measurements.
Cell line | Malic Enzyme | ATP Citrate Lyase | ||
---|---|---|---|---|
Activity | % of Control | Activity | % of Control | |
832/13 | 77 ±10 (3) | 100 | 129 ±8 (8) | 100 |
CHS | 79 ±11 (3) | 103 | 137 ±12 (6) | 106 |
SCOT 68 | 99 ±9 (3) | 130 | 123 ±6 (4) | 95 |
SCOT 713 | 73±7 (3) | 95 | 134 ±13 (4) | 104 |
SCOT 1676 | 72 ±10 (3) | 93 | 104 ±11 (10) | 81 |
SCOT 1184 | 59 ±7 (3) | 77 | 106 ±3 (6) | 82 |