Figure 5.

Site-specific cholinergic lesions result in restricted depletion of cortical cholinergic innervation. Normal patterns of cholinergic innervation to PFC (A), motor cortex (B), and AUD (C). Injections of the SAP immunotoxin directly within the nucleus basalis/substantia innominata depletes cholinergic innervation to all 3 regions (D–F). Focal injections of the immunotoxin into either the PFC (G–I), motor cortex (J–L), or AUD (M–O) selectively depleted cholinergic innervation to the targeted region but did not affect innervation to nontargeted regions. Scale bar in (O) = 50 μm and applies to all panels.