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. 2010 Aug 12;19(21):4216–4228. doi: 10.1093/hmg/ddq340

Figure 5.

Figure 5.

No change in cell density or cell proliferation markers in the primary somatosensory cortex or unaffected thalamic nuclei in severe SMA mice. (A) Representative confocal micrographs showing TOPRO3-labelled nuclei of all cells in the primary somatosensory cortex (S1BF) from wild-type (WT; left panel) and Smn−/−;SMN2 (right panel) mice at late-symptomatic (P5) ages. No qualitative differences in cell density were observed in Smn−/−;SMN2 mice. (B) Bar chart (mean ± SEM) showing cell density measured in the S1BF region (layers IV and V) of the brain in wild-type (+/+) and Smn−/−;SMN2 (−/−) mice at P5. There was no significant difference between the genotypes (ns P > 0.05; unpaired t-test. n = 4 mice per genotype). (C) Representative confocal micrographs showing TOPRO3-labelled nuclei of all cells in non-affected VPM/VPL thalamic nuclei from wild-type (left panel) and Smn−/−;SMN2 (right) mice at late-symptomatic (P5) ages. No qualitative differences in cell density were observed in Smn−/−;SMN2 mice. (D) Bar chart showing cell density measured in the thalamus in wild-type (+/+) and Smn−/−;SMN2 (−/−) mice at P5. There was no significant difference between the genotypes (ns P > 0.05; unpaired t-test; n = 4 mice per genotype). Scale bar = 35 µm.