Table 2.
Neuronal defects observed in kismet MARCM mutants
| Neuron | Gal4-line | n | Defects observed | % | P-value |
|---|---|---|---|---|---|
| γ neurons, MB | 201Y | ||||
| control (pupal) | 8 | Unpruned axons | 0% | — | |
| kis mutant (pupal) | 10 | Unpruned axons | 40% | <0.05 | |
| α, β, γ neurons, MB | OK107 | ||||
| Control (adult) | 9 | Lobe structure | 0% | — | |
| Cell migration | 0% | — | |||
| Axon migration | 11% | — | |||
| kis mutant (adult) | 11 | Lobe structure | 55% | <0.05 | |
| Cell migration | 36% | <0.05 | |||
| Axon migration | 55% | <0.05 | |||
| DCNs | Atonal | ||||
| Control (larval) | 9 | Cell position | 0% | — | |
| Axon migration | 0% | — | |||
| Cells out of cluster | 0% | — | |||
| Control (adult) | 6 | Axon extension (13.5 axons in medulla) | 0% | — | |
| kis mutant (larval) | 19 | Cell position | 53% | <0.001 | |
| Axon migration | 32% | <0.05 | |||
| Cells out of cluster | 32% | <0.05 | |||
| kis mutant (adult) | 6 | Axon extension (5.5 axons in medulla) | 100% | <0.001 |
Quantification of defects observed in different kismet mutant neurons. MB, mushroom body. Unpruned axons refers to the number of clones with unpruned γ axons extending into the α lobe at 18–20 h APF. Lobe structure indicates missing or altered lobe morphology in α, β or γ lobes. Cell position indicates the number of soma that appear lateral to their appropriate locations by the developmental time indicated. Axon migration indicates either axons crossing the midline for adult Kenyon cells or commissural axons failing to cross the midline or failing to migrate to their proper contralateral targets in larval DCNs. Axon extension indicates the presence of axons failing to extend from lobulla into the medulla in adult brains. P-values are indicated from a two-tailed Student's t-test comparing kis mutant with control.